ddpcr platform  (Bio-Rad)

Journal: Scientific Reports

Article Title: Comparing the precision of two digital PCR applications for copy number comparisons in protists

doi: 10.1038/s41598-025-13143-8

Figure Lengend Snippet: Limit of quantification (LOQ) for dPCR (blue) and ddPCR (red). The dashed black horizontal line represents the 0.35 coefficient of variation (CV) that was used to calculate the LOQ. Dashed blue vertical line represents the LOQ for dPCR (1.35) and dashed red vertical line represents LOQ of ddPCR (4.26).

Article Snippet: In this study, we compared the performance of the QX200 droplet digital PCR (ddPCR) platform from Bio-Rad (Temse, Belgium) with the QIAcuity One nanoplate digital PCR (ndPCR) from QIAGEN (Hilden, Germany) using DNA extracted from increasing cell numbers of the model ciliate Paramecium tetraurelia ( P. tetraurelia ) as well as from synthetic oligonucleotides.

Techniques:

Journal: Scientific Reports

Article Title: Comparing the precision of two digital PCR applications for copy number comparisons in protists

doi: 10.1038/s41598-025-13143-8

Figure Lengend Snippet: Relationship between log-transformed expected gene copy number per µl DNA calculated from Eq. and log-transformed measured gene copy number per µL DNA resulting from digital PCR experiments. Red data points and regression line indicates ddPCR results and blue indicates dPCR results. For both dataset a 3rd degree polynomial model was fitted.

Article Snippet: In this study, we compared the performance of the QX200 droplet digital PCR (ddPCR) platform from Bio-Rad (Temse, Belgium) with the QIAcuity One nanoplate digital PCR (ndPCR) from QIAGEN (Hilden, Germany) using DNA extracted from increasing cell numbers of the model ciliate Paramecium tetraurelia ( P. tetraurelia ) as well as from synthetic oligonucleotides.

Techniques: Transformation Assay, Digital PCR

Journal: Scientific Reports

Article Title: Comparing the precision of two digital PCR applications for copy number comparisons in protists

doi: 10.1038/s41598-025-13143-8

Figure Lengend Snippet: Boxplots showing coefficient of variation of technical replicates for dPCR (blue) and ddPCR (red) for different cell numbers of P. tetraurelia using (A) EcoRI or (B) HaeIII as a restriction enzyme in the assays.

Article Snippet: In this study, we compared the performance of the QX200 droplet digital PCR (ddPCR) platform from Bio-Rad (Temse, Belgium) with the QIAcuity One nanoplate digital PCR (ndPCR) from QIAGEN (Hilden, Germany) using DNA extracted from increasing cell numbers of the model ciliate Paramecium tetraurelia ( P. tetraurelia ) as well as from synthetic oligonucleotides.

Techniques:

Journal: Scientific Reports

Article Title: Comparing the precision of two digital PCR applications for copy number comparisons in protists

doi: 10.1038/s41598-025-13143-8

Figure Lengend Snippet: (A) Linear regression between dPCR and ddPCR for log-transformed copy number comparison of a 10-fold dilution series. ( B ) Linear regression between dPCR and ddPCR for copy number comparison of different cell numbers of P. tetraurelia separated by restriction enzyme.

Article Snippet: In this study, we compared the performance of the QX200 droplet digital PCR (ddPCR) platform from Bio-Rad (Temse, Belgium) with the QIAcuity One nanoplate digital PCR (ndPCR) from QIAGEN (Hilden, Germany) using DNA extracted from increasing cell numbers of the model ciliate Paramecium tetraurelia ( P. tetraurelia ) as well as from synthetic oligonucleotides.

Techniques: Transformation Assay, Comparison